The best Side of attune flow cytometer
The best Side of attune flow cytometer
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Data-enriched characterization of cell populations. Morphological data from images can include towards the richness of flow cytometry facts. For instance, the figure demonstrates an or else typical apoptosis assay making use of Annexin V and PI, incorporating mobile imaging to characterize cells in Every single populace to reveal morphologically unique functions. These insights couldn't are already acquired from multiplex staining on your own.
As samples are acquired over the Attune CytPix Flow Cytometer, the significant-speed brightfield camera captures and merchants illustrations or photos of detected occasions at a fee approximately six,000 pictures for each next, depending on the flow price and picture measurement. For better adaptability, Attune Cytometric Software package allows you to adjust the graphic capture frequency as essential and select a particular gate for image capture, if essential.
(B) RAW 264.seven macrophages are often annotated inaccurately with respect to particle count due to their complexity and noncircular borders. The quantity of singlets available for Assessment increases once the user trains and employs a far more accurate design.
The award-winning Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing with a substantial-pace brightfield camera for simultaneous substantial throughput flow cytometry and superior resolution brightfield imaging.
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Here, a seasoned user has gated singlets confidently. Following assessing the manual singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate includes more than four% aggregates.
Pair your Attune flow cytometer with the autosampler for enhanced workflow effectiveness, and add robotic integration for optimum throughput.
Our experts will design and style panels for you personally as persistently you need—free of charge, no item invest in desired.
Early signs of this contamination experienced at first been dismissed as particles. Backgating to discover new populations. Backgating imaged cells on the Attune CytPix Flow Cytometer also means that you can use morphological attributes to discover fascinating subpopulations that could not be obvious from flow cytometry information by yourself.
Perhaps most importantly, these situations have cells of clearly diverse phenotypes which could lead on to incorrect conclusions relating to double good events (particularly in rare populations).
AI-driven automatic impression analysis With all the Attune Cytometric Software package translates party features into unique morphology parameters that could be coupled with conventional fluorescence and scatter parameters to reveal very reproducible inhabitants changes and distinctive subsets.
We Earlier demonstrated the strength of imaging Motor vehicle-T/Ramos cell interactions. Allow’s check out just the inhabitants of greatest fascination, the double constructive events, to learn more. We are able to now use prolonged morphological parameters (here, circularity and skewness of intensity) to even further analyze the features of these populations and refine gating on these occasions, rising data robustness.
Leverage the strength of Superior technology and innovation to speed up your discoveries. Believe in within the Attune Flow Cytometer family members to provide the reliability, velocity, and accuracy that set your operate aside.
No clean/no lyse leukocyte Evaluation with imaging. Cells were acquired from 24-hour-old blood by dilution in one mM EDTA ( Professional UX/UI enables consumers to teach AI types for expanded applications. (A) The “Educate Design” characteristic provides annotation tools and true-time design training precision.
At the heart of each Attune Flow Cytometer lies our groundbreaking acoustic concentrating technologies, which makes use of sound waves to meticulously align cells in the flow cell. This revolutionary strategy surpasses standard hydrodynamic techniques, leading to more rapidly processing speeds, Fantastic knowledge good quality, and increased sample throughput.
Because the cell line had Beforehand been analyzed to the Attune CytPix Flow Cytometer, the scientists went again to the photographs more info and were able to doc the microbial infection at least five days before.
Agilent Value Assure assures you at the very least a decade of instrument use through the date you buy. In any other case, We are going to credit rating you with the residual price of the system toward an upgraded design.
What appeared to be a homogeneous populace is definitely extra numerous—an Perception that ought to be regarded when interpreting final results.